ScRNA-Seq

Last updated: Nov 15th, 2021

Base-Calling

Codes
  • Prepare sample sheet file
  • Run cellranger mkfastq

    cellranger mkfastq --id= <output_name> \
                       --run= <path_to_data_folder> \
                       --csv= <samplesheet.csv>


  • References
  • cellranger mkfastq
  • Count

    Codes
  • Run cellranger count

    cellranger count --id= <output_name> \
                     --fastqs= <path_to_fastq_folder> \
                     --sample= <Sample_name> \
                     --transcriptome= <path_to_ref_folder>


  • References
  • cellranger count
  • Aggregation

    Codes
  • Prepare aggregation sheet file
  • Run cellranger aggr

    cellranger aggr --id= <output_name> \
                    --csv== <aggr-sheet.csv> \
                    --normalize=mapped


  • References
  • cellranger aggr
  • Visualization

    Seurat

    References
  • Seurat